Evolution is constrained by earth’s natural history. Existing biological systems are rarely evolved to produce pure compounds in large quantities comparable to industrial yields. Instead, life evolved exquisite control over producing a wide range of chemical products. By interfacing inorganic catalysts with engineered bacteria, it may be possible to merge high industrial yields with biology’s chemical catalog. To this end, we created the ‘bionic leaf’, a system in which the bacterium Ralstonia eutropha converts CO2 and H2 produced from electrolysis into fusel alcohols and biomass. We are interested in optimizing this system’s yields along with tackling difficult chemical transformations.
Nangle SN, Sakimoto KK, Silver PA, Nocera DG. (2017). Biological-inorganic hybrid systems as a generalized platform for chemical production. Curr Opin Chem Biol. 41:107-113. doi: 10.1016/j.cbpa.2017.10.023. Link
Liu C, Sakimoto KK, Colon BC, Silver PA, Nocera DG. (2017). Ambient nitrogen reduction cycle using a hybrid inorganic-biological system. Proc Natl Acad Sci US A. 114(25):6450-6455. doi: 10.1073/pnas.1706371114. Link
Liu C, Nangle SN, Colon BC, Silver PA, and Nocera DG. (2017). C-Labeling the carbon-fixation pathway of a highly efficient artificial photosynthetic system. Faraday Discuss. 198:529-537. doi: 10.1039/c6fd00231e. Link
Water splitting-biosynthetic system with CO₂ reduction efficiencies exceeding photosynthesis. Liu C, Colón BC, Ziesack M, Silver PA, Nocera DG. Science. PMID: 27257255
Torella JP, Gagliardi CJ, Chen JS, Bediako DK, Colón B, Way JC, Silver PA, Nocera DG. (2015). Efficient solar-to-fuels production from a hybrid microbial-water-splitting catalyst system. Proc Natl Acad Sci U S A. 112(8), 2337-42. PMID: 25675518
Researchers: Marika Ziesack, Shannon Nangle, and Kelsey Sakimoto (postdoctoral fellows) in collaboration with Dan Nocera (Professor of Chemistry and Chemical Biology).